BN II System
Confidence in plasma protein results

BN II System
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BN II System

BN II System

BN II System connectivity to Aptio® Automation video

The BN™ II System is an easy-to-use, reliable nephelometric analyzer that offers a broad range of protein assays. With assays available across multiple sample types, you can conveniently assess numerous disease states such as gammopathies, kidney diseases, neurology, and chronic alcohol abuse—on just one system.

  • Designed for mid- to high-volume plasma protein throughput
  • Broad menu from one source; more than 60 assay protocols available for determination of various sample types
  • Up to 100 samples onboard
  • Antigen-excess security for confidence in results
  • Connectivity options to Aptio® Automation and FlexLab Automation solutions
  • Assistance with clinical interpretation through our innovative range of assessment programs available for Protis® Data Management System1

Features & Benefits

As the first dedicated protein analyzer that can be connected to lab automation, the BN™ II System provides flexible options to meet the needs of the mid- to high-volume plasma protein lab. It uses nephelometric technology for protein determination and supports the clinical management of a variety of disease states, such as gammopathies, kidney diseases, neurology, and chronic alcohol abuse.

Increased workflow efficiency:

  • Fully automated assay processing: from reading of sample tube bar codes to reporting of results
  • Average throughput up to 130 tests per hour2, and its high onboard capacity of up to 100 samples and 35 reagents provides a walkaway time of more than 2 hours
  • Wide measuring ranges enable fewer repeats
  • User-friendly software facilitates easy operation
  • Bidirectional host interface for convenient transfer of assay requests and results to the laboratory information system (LIS)

Flexible, efficient operation

  • Continuous loading and reloading of samples and/or reagents without interrupting the system’s current workload
  • Random-access processing eliminates sample batching
  • Accommodates standard sample tubes as well as micro cups for low-volume samples, such as samples from pediatric patients

Confident, consistent results

  • Confidence in results when analyzing samples with extremely high analytical concentrations
  • Optimized reaction conditions and assay-specific, automated pre-reaction protocols ensure valid results without operator intervention
  • Specimen level detection and positive barcode identification for samples, reagents, standards and controls to reduce human error

Improved workflow efficiency through automation

  • Ability to connect to Aptio® Automation and FlexLab Automation solutions
  • Interface module transfers sample tubes from the track into the BN II racks and pushes the racks onboard the analyzer
  • Reduces manual interaction
  • Extends walkaway capability
  • Continuous access to reagents and consumables
  • Enables manual loading of specialty samples, when needed

Innovative assay menu

  • Broad menu available from one source; over 60 assay protocols for management of various clinical indications
  • Routine and specialty assay consolidation
  • Innovative markers including monoclonal kappa and lambda free light chains (FLC), cystatin C, beta-trace protein (BTP), and carbohydrate-deficient transferrin (CDT)

Enhanced result reporting with PROTIS System

  • PROTIS® Assessment Software1 assists with interpretation support for a wide range of clinical indications
  • PROTIS System data-management software manages online data transfer between the BN II System and LIS
  • All data at a glance including graphical presentation of results, calculation of specific algorithms, and result interpretation suggestions modifiable by the user


Polyclonal and Monoclonal Gammopathies/ Immune System
FLC kappa
FLC lambda
Ig/Light Chain, type kappa
Ig/Light Chain, type lambda
IgG subclasses 1-4

Kidney Disease
α1-Microglobulin urine
α2-Macroglobulin urine3
β2-Microglobulin urine
β-trace protein3
Albumin urine
Cystatin C serum
FLC kappa urine
FLC lambda urine
Ig/Light Chain, type kappa urine3
Ig/Light Chain, type lambda urine3
IgG urine
Transferrin urine

α1-Acid Glycoprotein
High-Sensitivity CRP(CardioPhase® hsCRP)

Autoimmune/Rheumatoid Diseases
ADNase B

Cardiovascular Risk/Acute Cardiac Care
Albumin urine
Apo A-I
Apo B
Cystatin C
High- Sensitivity CRP (CardioPhase® hsCRP)

Chronic Alcohol Abuse
Transferrin (for %CDT calculation)

Allergic Diseases


Nutritional Assessment
RBP (retinol-binding protein)

Coagulation Disorders

Anemia/Iron Metabolism

Complement Activity
C1 Esterase Inhibitor

Blood-CSF Barrier Dysfunction
Albumin CSF
β-trace Protein3
FLC kappa CSF3
FLC lambda CSF3

Other Specialty Analytes
α2-Macroglobulin serum
Apo A-II3
Apo E3

Technical Specifications

Measuring principle Nephelometry; measurement of the scattered light intensity in a fixed angle of 13 - 24 degrees
Methods More than 60 programmed assay protocols
Sample throughput Effective: Approx. 130 tests/hour depending on the assay mix
Nominal: 225 tests/hour
Analysis method Fixed-time kinetics, end-point measurement, VLin Integral
Calibration Multi-point calibration
Rack transport unit Racks for up to 8 standard or control vials
Racks for up to 7 reagent vials
Racks for up to 10 sample tubes
Dilution unit 2 frames for max. 264 dilution cups
Size of sample tubes Diameter: 12 - 16 mm
Height: 55 - 100 mm
For pediatric samples:
Conical microtubes with a maximum filling volume of 1.5 mL
Barcode types Automatic reading of different barcode types:
2/5 interleaved
Code 39
Code 93
Code 128
Reagent volume 40 µL reagent consumption on average
Sample dilution 1 : 1 to 1 : 40,000
Level detection For samples, standards, controls, reagents, and system liquid containers
Reaction cuvettes 60 reusable cuvettes
Measuring temperature 37 ± 1.5°C
Light source Infrared high performance LED
Wavelength 840 ± 25 nm
Detector Photodiode with integrated pre-amplifier
Instrument dimensions and weight
Analyzer dimensions (W x H with tubings x D)
124 x 92 x 63 cm (49 x 36 x 25 in.)
Computer dimensions (W x H x D)
Approx. 19.7 x 19.7 x 3.6 cm (7.8 x 7.8 x 1.4 in)
Depth with keyboard Approx. 74 cm (29 in.)4
Printer dimensions (W x H x D)
Approx. 41 x 27 x 37 cm (16 x 11 x 15 in.)4
Total bench length Approx. 230 cm (90 in.)4
Analyzer weight 150 kg (330 lb.)
Terminal weight Approx. 7 kg (15.4 lb.)4
Room environment Ambient temperature 
15 to 32 °C (59 to 89 °F) 
Relative humidity 
Maintain between 30% and 85% (noncondensing) 
Average thermal output 
340 BTU/hr (100 W) in standby mode 
975 BTU/hr (286 W) in operating mode 
Water requirements 
Deionized water, NCClS Type 2 
Microbial count must not exceed 100 CFU/ml 
Electrical installation requirements Voltage
90–30 V
190–260 V
Power consumption
<140 VA (stand-by-mode)
<400 VA (operating mode)
Host interfacing
Download and host-query mode
Computer and Printer Terminal 
Apple Mac mini Computer
Lexmark E260 D or DN or equivalent
Connectivity RS232 bi-directional host interface
Aptio® Automation and FlexLab Automation solutions
CentraLink® Data Management System


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Related Products, Services & Resources

1. PROTIS Data Management System and PROTIS Assessment Software are not available for sale in the U.S.
2. Depending on the assay mix
3. Not available for sale in the U.S.
4. Dependent on model

The products/features (mentioned herein) are not commercially available in all countries. Due to regulatory reasons their future availability cannot be guaranteed. Please contact your local Siemens organization for further details.