Man with blue tint and hemostasis simulation


Explore a solution that sets a new standard in von Willebrand factor activity determination.

The INNOVANCE® VWF Ac assay is a sensitive, reliable, and convenient test system for direct, WHO-standardized determination of von Willebrand factor (VWF) activity. It is used as an aid in the diagnosis and monitoring of congenital or acquired VWF deficiencies in patients with bleeding disorders or at risk for VWF deficiency. In addition, it can be used for monitoring VWF substitution therapy. It employs an advanced new technology, allowing the assay to mimic the way in which VWF binds to glycoprotein Ib (GPIb), the major VWF receptor protein on platelets. Because the recombinant receptor protein includes two gain-of-function mutations, the assay does not require ristocetin.

Features & Benefits


Recommended screening method

A functional assay is the preferred screening method for detecting quantitative as well as qualitative defects of von Willebrand factor.1 


Direct determination of VWF activity

The INNOVANCE VWF Ac assay directly determines activity of VWF. Due to the gain-of-function mutations, VWF binding to GPIb does not require ristocetin (VWF:GPIbM)2 and is therefore less susceptible to effects of certain polymorphism variants.


Excellent correlation to the gold-standard method

The INNOVANCE VWF Ac assay shows excellent correlation with the ristocetin cofactor-based BC von Willebrand Reagent assay. 


Improved VWF testing workflow

The assay employs a liquid, ready-to-use reagent that requires no stirring.

Technical Specifications

15 μL 

 Required sample volume on CS-2500 and CS-5100 Systems

4–600% of norm
Measuring range

16 hours
Onboard stability on CS-2500 and CS-5100 Systems

18 hours
Onboard stability on CN-3000 and CN-6000 Systems3

Within Device/Lab CV Control Plasma N on CN-3000 and CN-6000 Systems3

~100 tests

Number of tests per kit

The INNOVANCE VWF Ac assay is a turbidimetric, latex-based assay that employs a liquid, ready-to-use reagent. The assay consists of three components and can be ordered as a kit.

Reagent III

Reagent II

Reagent I

INNOVANCE VWF Ac assay kit

  1. Siemens Healthineers INNOVANCE VWF Ac reagent I

    Description: Buffer with polystyrene particles coated with anti-GPIb monoclonal antibodies

Sysmex CN-3000/6000 Systems

Available on CN-3000/6000 Systems3

INNOVANCE VWF Ac assay applications are available on BCS® XP, CN-3000 and CN-60003, CS-2500 and CS-5100, and CA-660 Systems3. For details on ordering information and an overview of the current testing portfolio, download a copy of the hemostasis reagent catalog.

Clinical Use


Diagnostic algorithm for patients suspected of VWD1

To correctly diagnose cases involving a bleeding anamnesis or a family history of von Willebrand disease and subsequently monitor patient response to therapy, physicians require a specific test for von Willebrand factor. A functional assay is the preferred screening method for detecting quantitative as well as qualitative defects of von Willebrand factor. For more details on VWD subtypes and management, recommended lab diagnostics, and the benefits of the INNOVANCE VWF Ac assay, download the brochure.

Photo of vWF Ag assay from Siemens Healthineers

vWF Ag assay from Siemens Healthineers

For the evaluation of von Willebrand disease, VWF activity assays can be combined with VWF antigen assays to detect and distinguish between quantitative and qualitative VWF deficiencies.1

The vWF Ag assay is an immunoturbidimetric assay that can be used on all Siemens Healthineers and Sysmex automated coagulation instruments. Explore the extended portfolio of analyzers and assays for the diagnosis of bleeding disorders and therapy monitoring.

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