EMIT II Plus 6-Acetylmorphine Assay

EMIT II Plus 6-Acetylmorphine Assay

Accurately screen for heroin use

The EMIT® II Plus 6-Acetylmorphine Assay accurately detects heroin use, without cross-reactivity to morphine, morphine metabolites, and many common analgesics. Siemens offers a convenient and effective way to selectively test for heroin use and enables your laboratory to adjust to changing testing requirements.

  • High specificity for 6-Acetylmorphine (6-AM)
  • No cross-reactivity to morphine, morphine metabolites, and common analgesics
  • Qualitative (10 ng/mL cut-off) and semi-quantitative results
  • Low analytical sensitivity of 1.1 ng/mL*
  • >99% agreement with the reference method (GC/MS)
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Features & Benefits

Maintain a high level of integrity in your drug testing program, with the addition of the Emit® II Plus 6-Acetylmorphine Assay to your current offering.

Over the past decade, the number of heroin confiscations has more than doubled.1 Furthermore, an increasing amount of morphine-type drugs are prescribed as analgesics that can cross-react with traditional opiate assays.

EMIT® II Plus 6-Acetylmorphine Assay - Features & Benefits

Can a rapid screen differentiate heroin use from prescribed or ‘over-the-counter’ medications?

The presence of the heroin metabolite 6-Acetylmorphine (6-AM) in urine specifically confirms the use of heroin. The new EMIT II Plus 6-Acetylmorphine Assay is intended for the qualitative and semi-quantitative determination of 6-AM in human urine, at a cut-off value of 10 ng/mL. This new assay meets the Substance Abuse and Mental Health Services Administration (SAMHSA) guidelines for Federal Workplace Testing.

 

The EMIT® II Plus 6-Acetylmorphine Assay provides:

  • High specificity for 6-Acetylmorphine (6-AM)
  • No cross-reactivity to morphine, morphine metabolites, and common analgesics
  • Qualitative (10 ng/mL cut-off) and semi-quantitative results
  • Low analytical sensitivity of 1.1 ng/mL*
  • >99% agreement with the reference method (GC/MS)
  • Rapid results: 11 minutes to first result
  • Convenient dual calibrator/control with the EMIT II Plus Ecstasy Assay

Applications are available for Siemens’ Viva-Jr®, Viva-E®, and V-Twin® Drug Testing Systems, the Dimension® and Dimension Vista®, as well as a variety of other general chemistry analyzers.

1. World Drug Report 2009, United Nations Office on Drugs and Crime (UNODC)
*Analytical sensitivity of 1.1 ng/mL on Vital Systems.

Technical Specificiations

 Assay Principle

  Homogeneous Enzyme Multiplied Immonoassay (EMIT)

 Cut-off

  10 ng/mL

 Assay range

  0-20 ng/mL

 Sample

  Urine  

 Reagent Stability

  Unopened until expiration date; Opened working reagent, 4 weeks

 Calibration Frequency

  Based on control results

 

Level

Mean

Repeatability

 

Within-Lab

 

 

 

(ng/mL)

SD

%CV

SD

%CV

Control 1

7.5 ng/mL

558

1.5

0.3

7

1.3

Cut-off

10.0 ng/mL

591

1.7

0.3

6.5

1.1

Control 2

12.5 ng/mL

622

1.7

0.3

6

1

 

Level

Mean

Repeatability

Within-Lab

 

 

 

 

(ng/mL)

SD

%CV

SD

%CV

Control 1

7.5 ng/mL

8.7

0.12

1.4

0.43

5.1

Cut-off

10.0 ng/mL

11.3

0.14

1.5

0.48

4.2

Control 2

12.5 ng/mL

14.2

0.16

1.1

0.5

3.5

lg

Concentrations of compounds that produce negative results vs. the 10 ng/mL cut-off

Compounds Concentration Tested

(ng/mL)

Buprenorphine

1,000,000

Codeine

500,000

Dextromethorphan

100,000

Dihydrocodeine

500,000

Heroin

80

Hydrocodone

300,000

Hydromorphone

100,000

Imipramine

200,000

Levorphanol

100,000

Meperidine

800,000

Morphine

100,000

Morphine-3-Glucuronide

600,000

Morphine-6-Glucuronide

600,000

Nalorphine

100,000

Naloxone

300,000

Naltrexone

300,000

Norcodeine

600,000

Normophine

100,000

Oxycodoen

400,000

Oxymorphone

100,000

9R129UL

EMIT® II Plus 6-Acetylmorphine Assay, large

R1=1000 mL and R2=500 mL

9R039UL

EMIT II Plus 6-Acetylmorphine Assay, small

R1=28 mL and R2=14 mL

9A509UL

EMIT Calibrator/Control Level 0

14 mL

9R529UL

EMIT II Plus 6-AM/Ecstasy Calibrator/Control Level 1

10 mL

9R549UL

EMIT II Plus 6-AM/Ecstasy Calibrator/Control Level 2

10 mL

9R569UL

EMIT II Plus 6-AM/Ecstasy Calibrator/Control Level 3

10 mL

9R589UL

EMIT II Plus 6-AM/Ecstasy Calibrator/Control Level 4

10 mL