BEP 2000 Advance SystemWhere convenience begins

The BEP 2000 Advance® System* and Enzygnost® and Novagnost® Assays (ELISA) help to detect infectious diseases (IDD), such as HIV, hepatitis, borreliosis and herpes but also bacteria, fungi, worms and other parasites. It provides fully automated processing of microtitration plates (MTP). BEP 2000 Advance together with Enzygnost and Novagnost offer:

  • Perfect design for low- to mid-volume labs or specialty IDD labs
  • Barcode identification of primary tubes and reagents
  • In-process control of all steps
  • User-friendly, convenient software
  • Broad menu of proven Enzygnost and Novagnost assays
Fale Conosco

Características e Benefícios

Automated ELISA processing
The BEP 2000 Advance® System is a fully automated microtitration plate (MTP) analyzer, perfectly designed for low- to mid-volume labs or specialty ID labs. It consolidates the laboratory workflow on one instrument with its broad menu of Enzygnost® and Novagnost® assays.

BEP 2000 Advance System

Advance Analyzer meets the demands for a high level of automation. It runs MTPs fully automated from sample to results and features a broad menu of infectious disease tests for the detection of HIV, hepatitis, borreliosis, herpes but also bacteria, fungi, worms and other parasites.

The system can process up to 4 MTPs simultaneously plus one MTP by reloading and up to 100 patient samples at the same time.

The requirements for the next generation of MTP-systems are included as well as newly developed unique software features. The well known and proven BEP concepts for GMP/GLP (good manufacturing practice / good laboratory practice) are also integrated in the BEP 2000 Advance Analyzer.

Convenience from the beginning
Designed for integration into your laboratory workflow, the BEP 2000 Advance Analyzer offers a sample-in / result-out process with online connection to your host computer. The specially designed front cover allows easy access to the individual components.

The BEP 2000 Advance System provides the following features and benefits:

Real automation for microtiter plates (MTP)

  • Processes up to 4 MTPs in one run and allows reloading of 1 additional MTP
  • Performs all assay steps: from reagent pipetting, through incubation, washing, reading to result evaluation
  • Records positive and negative events through in process control
  • Provides photometric reading and evaluation of results
  • Monitors the system status (accreditation) to increase safety and reliability
  • Reports qualitative and quantitative results (including alpha method, standard curves and user defined curves)

Integrated primary tube handling

  • Loads up to 100 primary tubes onboard – allows for increase up to 200 primary tubes by using the reloading option
  • Provides sample pre-dilution with Enzygnost and Novagnost assays and sample distribution from primary tubes
  • Allows positive identification of all samples and reagents through barcode identification

Disposable conductive tips to avoid carryover

  • Offers the choice of 2 different sizes of disposable tips (300µL and 1100µL) for single and multishot dispensing
  • Avoids carryover with specific hardware and software components designed and developed solely for the BEP 2000 Advance System
  • Allows clot detection

Flexible design

  • Runs round and flat bottom plates and allows the adaption of basically all ELISA MTPs
  • Comes with 37°C incubators with built-in shaker to provide homogeneous and accurate results: meets the demand for 3rd party assays

User-friendly, convenient software

  • Keeps training requirements at a minimum: it’s easy to learn and easy to use
  • Offers multiple software functions during the run
  • Monitors system status
  • Defines new work lists
  • Optimizes your laboratory workflow
  • Combines up to 12 tests / plate frame (same assay procedure)
  • Schedules loading time to increase efficiency
  • Provides bidirectional interface to a LIS (ASTM specification)
  • Downloads and uploads work list or results from and to LIS
  • Reduces operator errors and increases safety


Enzygnost® Assays

Virus - Retrovirus
HIV Integral 4

Virus - Hepatitis
HBsAg 6.0
HBsAg Confirmatory Test
Anti-HBc monoclonal
Anti-HBs II
Anti-HCV 4.0
HBe/Anti-HBe monoclonal

Lyme link VlsE/IgG
Anti-Helicobacter pylori/IgG
Anti-Helicobacter pylori/IgA

Anti-Rubella Virus/IgG
Anti-Rubella Virus/IgM
Anti-CMV/IgG + IgM

Virus - Others
Anti-Measles Virus/IgG
Anti-Measles Virus/IgM
Anti-Parotitis Virus/IgG
Anti-Parotitis Virus/IgM
Anti-TBE/FSME Virus (IgG, IgM)

CSF Application for:
Enzygnost Anti-Measles Virus/IgG
Enzygnost Anti-Rubella Virus/IgG
Enzygnost Anti-VZV/IgG
Enzygnost Anti-HSV/IgG
Enzygnost Anti-Parotitis Virus/IgG
Enzygnost Lyme link VlsE/IgG
Enzygnost Borreliosis/IgM

Novagnost® Assays

Bordetella pertussis IgG
Bordetella pertussis IgM
Bordetella pertussis IgA
Bordetella pertussis Toxin IgG
Bordetella pertussis Toxin IgA
Brucella IgG
Brucella IgM
Chlamydia trachomatis IgA
Chlamydia pneumoniae IgA
Chlamydia trachomatis IgG
Chlamydia pneumoniae IgG
Chlamydia trachomatis IgM
Chlamydia pneumoniae IgM
Coxiella burnetii (Q-Fever) Phase 1 IgG
Coxiella burnetii (Q-Fever) Phase 2 IgG
Coxiella burnetii (Q-Fever) Phase 2 IgM
Diphtheria Toxin IgG
Diptheria Toxin 5S IgG
Legionella pneumophila IgG
Legionella pneumophila IgM
Leptospira IgG
Leptospira IgM
Mycoplasma pneumoniae IgG
Mycoplasma pneumoniae IgM
Mycoplasma pneumoniae IgA
Tetanus Toxin IgG
Tetanus Toxin 5s IgG

Herpes simplex Virus 1 IgG
Herpes simplex Virus 1 IgM
Herpes simplex Virus 2 IgG
Herpes simplex Virus 2 IgM
Herpes simplex Virus 1+2 IgG
Herpes simplex Virus 1+2 IgM

Virus - Others
Adenovirus IgG
Adenovirus IgM
Chikungunya IgG
Chikungunya IgM
Dengue IgG
Dengue IgM
Influenza A IgG
Influenza A IgA
Influenza B IgG
Influenza B IgA
Parainfluenza 1-3 IgA
Parainfluenza 1-3 IgG
Parvovirus B 19 IgG
Parvovirus B 19 IgM

Aspergillus fumigatus IgG
Aspergillus fumigatus IgM
Candida albicans IgG
Candida albicans IgM
Candida albicans IgA

Chagas IgG
Echinococcus IgG
Entamoeba histolytica IgG
Leishmania infantum IgG
Schistosoma mansoni IgG

Ascaris lumbricoides IgG
Toxocara canis IgG
Trichinella spiralis IgG
Taenia solium IgG

Especificações Técnicas


Number of plates:

Up to 4 plates at once

Number of samples Max

100 tubes, up to max. φ 16 mm, length up to 200 mm

Number of reagents

Up to 4 reagent bottles in different sizes to be placed in special racks for Siemens reagents

Pre-dilution positions

Positions for up to 4 pre-dilution plates

Number of tips

Up to 5 tip racks for 300 μl or 1100 μl disposable tips


Continuous loading of plates, samples, reagents and tips


Spectral range

400 - 700 nm

Dynamic range

-0.100 to 3.000 O.D.


+/- 0.005 or 2.5%


0 - 2.000 O.D. +/- 1%


Photo diode

Reading time

< 15 seconds

Read modes

OD and Kinetic mode


Up to 8 positions

Pipetting System


Liquid pipettor for disposable tips

Liquid level detection

Standard setting

Volumes min. / max.

Small tips: 10–300 μL / large tips: 1000 μL


<-15% at 25 μL (aqua)

<-5% at 100 μL (aqua)


<5% CV at 25 μL

<2.5% CV at 100 μL


Tip detection, mixing, multi-dispensing mode



4 independent chambers

Temperature range

Minimum temperature = RT + 5 °C

Maximum temperature = 50 °C


+/- 1 °C mean of plate


+/- 0.7 °C across plate



Max. 3 positions wash buffer + 1 distilled water

Wash head

1 x 8 with verify option

Dispense volume

200 - 2500 μl/well


+/- 5% CV at 300 μl

Residual volume

< 2.5 μl in U-shaped bottom

< 4 μl in flat bottom

Fluid alarms

Low reagent, waste full

Features speeds

Sweeps, soak, purge, top & bottom wash, variable pump

Bar Code


Bar code identification of samples, reagents and test plates possible

Personal computer


Microsoft Windows® - compatible

Software features

Operating system

Microsoft Windows® 95, Microsoft Windows® 2000 or Microsoft Windows® 7


ASTM interface

Flexible ASCII import of worklist sample ID's

Time Scheduling Schedules

4 plates with user options

Multiple assays per plate

Up to 12 assays per plate

Export options

Flexible ASCII export and report


User definable result classes


Linear to quadratic regression, sigmoidal, and many more

Language support

Multiple languages possible

QA analysis

Mean, SD, CV, Standard Error and Levey-Jennings

Power Supply

Universal a.c. input

100 - 260 VAC, 47 - 63 Hz

Typically max. 500 VA

Main fuses

4 A T


W x D X H

114 cm x 156 cm x 100 cm

(Depth with waste bag and completely opened drawer)



130 kg



67 dB (distance 1 m)

Environmental conditions

This standard applies equipment designed to be safe at least under the following conditions:

 indoor use

altitude up to 2000 m

temperature 5 °C to 40 °C

maximum relative humidity 80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40 °C

mains supply voltage fluctuations up to +/- 10% of the nominal voltage

applicable rated pollution degree

 Operating conditions

15 °C to 25 °C